Anti-SENP1 Antibody Picoband™ (monoclonal, 5F4)

Product Name

Anti-SENP1 Antibody Picoband™ (monoclonal, 5F4)

View all SENP1 Antibodies

SKU/Catalog Number

M02156-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SENP1 Antibody Picoband™ (monoclonal, 5F4) catalog # M02156-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-SENP1 Antibody Picoband™ (monoclonal, 5F4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02156-1)

Host

Mouse

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Monoclonal

Clone Number

5F4

Isotype

Mouse IgG1

Immunogen

E.coli-derived human SENP1 recombinant protein (Position: N19-P619).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M02156-1 is reactive to SENP1 in Human

Applications

M02156-1 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

73 kDa

Calculated molecular weight

73.481kDa

Background of SENP1

Sentrin-specific protease 1 is a protein that in human is encoded by the SENP1 gene. This gene is mapped to 12q13.11. This gene encodes a cysteine protease that specifically targets members of the small ubiquitin-like modifier (SUMO) protein family. This protease regulates SUMO pathways by deconjugating sumoylated proteins. This protease also functions to process the precursor SUMO proteins into their mature form. Alternate splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry, 1-3 μg/1x10⁶ cells, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of SENP1 using anti-SENP1 antibody (M02156-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Raji whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human U87 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SENP1 antigen affinity purified monoclonal antibody (Catalog # M02156-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for SENP1 at approximately 73 kDa. The expected band size for SENP1 is at 73 kDa.

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Figure 2. Flow Cytometry analysis of K562 cells using anti-SENP1 antibody (M02156-1).
Overlay histogram showing K562 cells stained with M02156-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-SENP1 Antibody (M02156-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For SENP1 (Source: Uniprot.org, NCBI)

Alternative Names

EC 3.4.22; EC 3.4.22.-; SENP1; Sentrin/SUMO-specific protease SENP1; sentrin-specific protease 1; SUMO1/sentrin specific peptidase 1; SUMO1/sentrin specific protease 1; SuPr-2 SENP1 SuPr-2 SUMO specific peptidase 1 sentrin-specific protease 1|SUMO1/sentrin specific peptidase 1|SUMO1/sentrin specific protease 1|sentrin/SUMO-specific protease SENP1

For more info on SENP1, check out the SENP1 Infographic

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In this infographic, you will see the following information for SENP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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